3D reconstruction of histological sections: Application to mammary gland tissue

TLDR

The study introduces a novel method for automatically reconstructing 3D models of thick tissue blocks from 2D histological sections. The method employs a high‑content, multiscale imaging system that integrates image acquisition, annotation, registration, and 3D reconstruction, and is validated on synthetic and real data. The authors successfully reconstructed an entire mouse mammary gland in 3D and integrated high‑resolution molecular data into the model. Published in Microsc.

Abstract

Abstract In this article, we present a novel method for the automatic 3D reconstruction of thick tissue blocks from 2D histological sections. The algorithm completes a high‐content (multiscale, multifeature) imaging system for simultaneous morphological and molecular analysis of thick tissue samples. This computer‐based system integrates image acquisition, annotation, registration, and three‐dimensional reconstruction. We present an experimental validation of this tool using both synthetic and real data. In particular, we present the 3D reconstruction of an entire mouse mammary gland and demonstrate the integration of high‐resolution molecular data. Microsc. Res. Tech. 73:1019–1029, 2010. © 2010 Wiley‐Liss, Inc.