Publication | Open Access
An improved method for isolating cardiac myocytes useful for patch-clamp studies.
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1990
Year
Tissue EngineeringCardiac MuscleImproved MethodEngineeringCardiac Progenitor CellsBiomedical EngineeringCellular PhysiologyMatrix BiologyCardiologyCardiac MechanicCardiovascular ImagingMechanobiologyCardiomyopathyInitial DispersionCardiac MyocytesCell BiologyG Omega SealCardiac PhysiologyPhysiologyElectrophysiologyCardiovascular PhysiologyPatch-clamp StudiesMedicineExtracellular Matrix
An improved method for isolating cardiac myocytes is described. This method consists of the initial dispersion of the myocytes with a new type of collagenase and the following treatment of the cells with a non-specific protease. This method gives more than 80% rod-shaped, viable, and quiescent cells, which are suitable for biochemical as well as electrophysiological experiments. Formation of a G omega seal and subsequent whole-cell recording is easily performed using these cells.