Cytochrome c: A Biochemistry Laboratory Course

Abstract

A unified biochemistry laboratory course has been developed. Rather than using miscellaneous laboratory experiments, the concept behind this course is to focus on a theme: the process of biochemical research. The laboratory deals entirely with one protein, cytochrome c, simulating the experience of a real-world research project. The laboratory is built around team-learning: groups of students isolate cytochrome c, characterize it by a variety of biochemical and bioinorganic methods, attempt to determine its function, and finally alter the protein to produce a modifier of oxidative nucleic acid damage. In this way, the students are introduced in general terms to the techniques necessary to research a new biochemical problem. Overall, by exposing students to the excitement of research problems, it is hoped their interests in pursuing careers in chemical or biochemical research will be stimulated.