Production of Active Human Carbonic Anhydrase II in E. Coli. - Concepedia

Concepedia

Publication | Open Access

Production of Active Human Carbonic Anhydrase II in E. Coli.

DOI Full Paper Access

80

Citations

0

References

1988

Year

Cecilia Forsman, Gity Behravan, A.L. Osterman, Bengt‐Harald Jonsson, Curt R. Enzell, Jan-Eric Berg, Mihály Bartók, István Pelczer, György Dombi

Acta chemica Scandinavica/Acta chemica Scandinavica. B, Organic chemistry and biochemistry/Acta chemica Scandinavica. A, Physical and inorganic chemistry/Acta chemica Scandinavica. Series B. Organic chemistry and biochemistry/Acta chemica Scandinavica. Series A, Physical and inorganic chemistry

E. ColiMolecular BiologyEscherichia ColiMicrobial PhysiologyMolecular GeneticsProtein SynthesisBiosynthesisBiological Carbon FixationBioenergeticsBiochemical EngineeringGene StructureBiotransformationBiochemistryMolecular MicrobiologyGene ExpressionProtein BiosynthesisCellular EnzymologyNatural SciencesBiotechnologyTotal ProteinMicrobiologyTac PromoterMedicineCarbonyl Metabolism

Abstract

cDNA encoding human carbonic anhydrase II has been isolated and its nucleotide sequence determined. Expression of the isolated carbonic anhydrase gene in Escherichia coli from a plasmid containing the tac promoter yielded an active enzyme at a level of about 1% of total protein.