Ankara Üniversitesi Veteriner Fakültesi Dergisi · 2010 · 18 citations · 15 references
Veterinary MedicineInfectious DiseasesVeterinary MicrobiologyDiagnostic MicrobiologyMolecular VirologyVirologyAnimal Infectious DiseasesAnimal VirusesLivestock Infectious DiseasesVeterinary Diagnostic MicrobiologyVeterinary VirologyViral DiagnosticsBovine VirologyImmunofluorescence AssaysImmunofluorescence DiagnosisImmunofluorescence StainingLivestock Viral DiseasesLivestock VirologyVirological Diagnosis
To investigate the occurrence of bovine herpesvirus type 4 (BoHV-4) infection in a dairy herd including cows with metritis, various methods were used for the diagnosis BoHV-4 in vaginal discharge samples. Blood serum samples were obtained from 368 cows for serodiagnosis of BoHV-4 infection. Of 368 serum samples, 256 (69.6%) were found positive for BoHV4 specific antibodies. For the virological study, vaginal discharge samples of 55 cows with metritis were used and tested for BoHV-4 using polymerase chain reaction (PCR), virus isolation and immunofluorescence techniques. Twenty-nine percent (16/55) of the vaginal discharge samples obtained from cows with metritis were found positive for BoHV-4 DNA by PCR. In this study, the presence of BoHV-4 was determined in cows with metritis in post-partum period in a dairy herd
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