A Study of the Fibrin Plate Assay of Fibrinolytic Agents

Abstract

The reproducibility, precision and required conditions of the fibrin plate method for determination of fibrinolytic agents are described in detail. Under optimal conditions the assay is a sensitive and precise method for the quantitative determination of fibrinolytic agents. Thus, with a tissue plasminogen activator preparation its sensitivity is approximately 0.02 CTA urokinase units/ml, and dilutions yielded a curve with a slope (double-logarithmic) with a day-to-day mean of 0.53 with a coefficient of variation of 5.1%.