Grapevine Fanleaf Virus Monoclonal Antibodies: their Use to Distinguish Different Isolates

Abstract

Abstract Monoclonal antibodies (MCAs) specific for Grapevine Fanleaf Virus (GFV) were obtained by fusion of a non‐secreting myeloma cell line with the spleen cells from Balb/c mice immunized with GFV isolate F 13. The MCAs were tested with apanel of GFV isolates, using both crude extracts and purified virus particles. Some of the MCAs recognize all 41 isolates tested and are suitable as a diagnostic reagent for GFV detection. Certain MCAs do not bind to some of the isolates, while others show a lower reactivity for certain isolates than for the immunogen F 13. MCAs make it possible to distinguish GFV isolates which until now could not be distinguished by using polyclonal antiserum.