Colorimetric Assay for Lead Ions Based on the Leaching of Gold Nanoparticles

TLDR

Au nanoparticles form Au(S₂O₃)₃³⁻ complexes with thiosulfate, producing slight decreases in surface plasmon resonance absorption. The probe exploits Au nanoparticles that leach rapidly when Pb²⁺ is present with thiosulfate and 2‑mercaptoethanol, enabling a colorimetric, label‑free detection. It achieves a 0.5 nM limit of detection, 1000‑fold selectivity, a 2.5 nM–10 µM linear range, and accurately quantifies Pb²⁺ in soil and river samples, with SALDI‑TOF confirming Pb‑Au alloy formation and accelerated dissolution.

Abstract

A colorimetric, label-free, and nonaggregation-based gold nanoparticles (Au NPs) probe has been developed for the detection of Pb2+ in aqueous solution, based on the fact that Pb2+ ions accelerate the leaching rate of Au NPs by thiosulfate (S2O32−) and 2-mercaptoethanol (2-ME). Au NPs reacted with S2O32− ions in solution to form Au(S2O3)23− complexes on the Au NP surfaces, leading to slight decreases in their surface plasmon resonance (SPR) absorption. Surface-assisted laser desorption/ionization time-of-flight ionization mass spectrometry (SALDI-TOF MS) data reveals the formation of Pb−Au alloys on the surfaces of the Au NPs in the presence of Pb2+ ions and 2-ME. The formation of Pb−Au alloys accelerated the Au NPs rapidly dissolved into solution, leading to dramatic decreases in the SPR absorption. The 2-ME/S2O32−-Au NP probe is highly sensitive (LOD = 0.5 nM) and selective (by at least 1000-fold over other metal ions) toward Pb2+ ions, with a linear detection range (2.5 nM−10 μM) over nearly 4 orders of magnitude. The cost-effective probe allows rapid and simple determination of the concentrations of Pb2+ ions in environmental samples (Montana soil and river), with results showing its great practicality for the detection of lead in real samples.