Further Effects of Nonsteroidal Anti-inflammatory Compounds on Blastocyst Hatching in vitro and Implantation Rates in the Mouse1

Abstract

When tested in protein-free medium NCTC 109, mouse blastocyst hatching in vitro was inhibited by low doses (<50 μM) of indomethacin, meclofenamic acid, mefenamic acid, and naproxen. Ketoprofen was not inhibitory within the same concentration range. In medium supplemented with 10% fetal calf serum, meclofenamic acid inhibited hatching above 100 μM but in contrast naproxen had no effect at 500 μM. By ultrafiltration plus HPLC analysis, it was found that for meclofenamic acid over the range 100–200 μM, 92–83% was bound to 10% fetal calf serum.