The Ratio Between Collagenase Class I and Class II Influences the Efficient Islet Release From the Rat Pancreas

Abstract

The present study demonstrates that the C-ratio is of significant relevance for the outcome after enzymatic rat islet isolation. The data indicate further that purified collagenase class I or class II does not damage islet tissue even if used in excess. The present study can serve as a start for subsequent experiments in the human pancreas.