Three-dimensional nano-HAp/collagen matrix loading with osteogenic cells in organ culture

TLDR

Transplantation of osteogenic cells with a suitable matrix, particularly in a three‑dimensional porous scaffold, is a key strategy for repairing large bone defects. The study aimed to develop three‑dimensional osteogenic cell/nano‑HAp/collagen constructs in vitro using organ culture and convolving techniques. A nano‑HAp/collagen composite sheet was convolved into a porous 3‑D scaffold, and organ culture techniques seeded osteogenic cells to create the tissue‑engineering construct. Scanning electron microscopy and histology revealed spindle‑shaped cells migrating and proliferating throughout the scaffold, adopting a tridimensional polygonal shape and synthesizing new bone matrix at the interface with bone fragments. © 1999 John Wiley & Sons, Inc., J Biomed Mater Res 44:407–415.

Abstract

Transplantation of osteogenic cells with a suitable matrix is one strategy for engineering bone tissue. Three-dimensional distribution and growth of cells within the porous scaffold are of clinical significance for the repair of large bony defects. A nano-HAp/collagen (nHAC) composite that mimics the natural bone both in composition and microstructure to some extent was employed as a matrix for the tissue engineering of bone. A porous nHAC composite was produced in sheet form and convolved to be a three-dimensional scaffold. Using organ culture techniques and the convolving method, we have developed three-dimensional osteogenic cells/nHAC constructs in vitro. Scanning electron microscopic and histological examination has demonstrated the development of the cells/material complex. Spindle-shaped cells migrating out of bone fragments continuously proliferated and migrated throughout the network of the coil. The porous nHAC scaffold provided a microenvironment resembling that seen in vivo, and cells within the composite eventually acquired a tridimensional polygonal shape. In addition, new bone matrix was synthesized at the interface of bone fragments and the composite. © 1999 John Wiley & Sons, Inc. J Biomed Mater Res, 44, 407–415, 1999.