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INHIBITION OF THE AROMATIZING ACTIVITY OF HUMAN PLACENTA BY SU 4885

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1964

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Abstract

It has been shown recently (Griffiths, 1963) that administration of 2-methyl-1: 2-bis-(3-pyridyl)-1-propanone (SU 4885) to golden hamsters decreases the steroid '19-hydroxylase' activity of the adrenal gland as well as that of the ' 11β-hydroxylase' on which SU 4885 was considered to have a specific inhibitory action (Liddle, Island, Lance & Harris, 1958). SU 4885 has also been shown by Kahnt & Neher (1962) to inhibit 19-hydroxylation in beef adrenal cortex homogenates. Since hydroxylation of the angular C-19 methyl group appears to be an essential stage in the biosynthesis of oestrogens (Meyer, 1955; Ryan, 1959; Longchampt, Gual, Ehrenstein & Dorfman, 1960), it was decided to ascertain whether SU 4885 has any effect on the steroid aromatizing enzyme system of human placental tissue. Fresh placentae were dissected free of foetal membranes and processed at 4°. Saline-washed tissue was minced, and 200 g. were homogenized in 65 ml. buffer containing 0·05 m-phosphate, (pH 7), 0·25 m-sucrose