Measurement of cytosolic free Ca2+ in individual small cells using fluorescence microscopy with dual excitation wavelengths

A new generation of Ca2+ indicators with greatly improved fluorescence properties.

Grzegorz Grynkiewicz, Martin Poenie, Roger Y. Tsien · Journal of Biological Chemistry · 1985 · 21.7K citations · Full text

Natural Sciences, Biochemistry, Chemistry +17

Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator.

Roger Y. Tsien, Tullio Pozzan, T J Rink · The Journal of Cell Biology · 1982 · 2.3K citations · Full text

Life Sciences, Biochemistry, Biophysics +13

A non-disruptive technique for loading calcium buffers and indicators into cells

Roger Y. Tsien · Nature · 1981 · 1.1K citations

Life Sciences, Biophysics, Cell Biology +12

Cytoplasmic pH and free Mg2+ in lymphocytes.

T J Rink, Roger Y. Tsien, Tullio Pozzan · The Journal of Cell Biology · 1982 · 1K citations

Life Sciences, Biochemistry, Physiology +13

Intracellular pH: Its Measurement, Regulation, and Utilization in Cellular Functions.

Levinson Bl · The Yale Journal of Biology and Medicine · 1982 · 549 citations

Life Sciences, Biochemistry, Cell Biology +12