Biological Letters · 2004 · 29 citations · 11 references
Life SciencesPlant BiologyPlant SciencesPlant Hormone BiologyPlant PhysiologyPlant PropagationAuxin BiologyCytokinin BiologyIn Vitro ConservationMedicinal Plant ProductionPlant EstablishmentPlant HormonesCulture Medium FormulationCytokinin Control Of Shoot DevelopmentCytokinin Effects On GrowthCytokinin ResponsesCytokinin-mediated GrowthCytokinin-mediated Shoot GrowthHormonal Regulation Of Shoot GrowthMedicinal Plant Management
In vitro shoot multiplication from nodal explants of Clitoria ternatea was standardized by using MS (Murashige & Skoog) medium supplemented with a cytokinin (BA or Kin). Inclusion of NAA in the culture medium along with BA promoted a higher rate of shoot multiplication. The mean number of shoots per explant after 4 weeks of culture was maximum (5.21) on the MS medium supplemented with 2.0 mg l -1 BA and 0.25 mg l -1 NAA. The elongated shoots rooted within 7-8 days in Ω strength MS medium supplemented with 0.25 mg l -1 of NAA and 2% (w/v) sucrose. About 85% of the rooted plantlets survived acclimatization and transfer to the greenhouse. The results will facilitate the conservation and propagation of this important medicinal plant.
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Neeti Jain, C.C Ohal, S.K Shroff et al. · Pharmacology Biochemistry and Behavior · 2003 · 194 citations