Diabetes Care · 1995 · 11 citations · 0 references
Life SciencesGeneticsMolecular GeneticsHuman GeneticsImmunogeneticsMedical GeneticsDiabetes MellitusDisease Susceptibility LociGenetic SusceptibilityGenotype-phenotype Association AnalysisHla MatchingHla TypingGenetic Risk MarkersGermline Susceptibility MarkersHla Allele PolymorphismHla AllelesHla Genotype AssociationsHla Polymorphism AnalysisHla Risk AllelesMolecular Hla Typing
OBJECTIVE: To investigate, in Senegalese subjects, the frequency of human leukocyte antigen (HLA)-DQ beta 1 alleles and their role in susceptibility to insulin-dependent diabetes mellitus (IDDM). RESEARCH DESIGN AND METHODS: HLA-DQ beta 1 typing was done in 55 IDDM subjects and 118 nondiabetic control subjects by means of polymerase chain reaction restriction fragment length polymorphism. RESULTS: Alleles bearing a codon for an Asp residue at position 57 in the DQ beta-chain were associated with a significantly lower risk of IDDM. Alleles 0201 and 0302 (Ala57) were positively associated with diabetes, but allele 0501 (Val57) was less frequent in IDDM subjects than in control subjects. CONCLUSIONS: HLA-DQ beta 1 alleles may be genetic susceptibility markers for IDDM in the Senegalese population, as they are in Caucasian populations.