Concepedia

Brain tubutin and actin cDNA sequence: isolation of recombinant plasmids

Irith Ginzburg, Annie de Baetselier, Michael Walker, L. Behar, Hans Lehrach, A.-M. Frischauf, Uriel Z. Littauer

Nucleic Acids Research · 1980 · 56 citations · 23 references

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Concepts

Abstract

Rat brain mRNA enriched for tubulin and actin sequences was used to prepare double stranded cDNA. A library of recombinant clones was constructed by inserting the dsDNA into the Pst1 site of pBR322 plasmid and transformation of E. coli chi 1776 host. Clones bearing sequences coding for tubulin and actin were identified and characterized.

References

23

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

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Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

Haider Raza, Annie John, Abderrahim Nemmar · Cellular Physiology and Biochemistry · 2013 · 47.3K citations · Full text

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A rapid alkaline extraction procedure for screening recombinant plasmid DNA

H.C. Birnboim, Janine Doly · Nucleic Acids Research · 1979 · 14.9K citations · Full text

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Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose

Haim Aviv, Philip Leder · Proceedings of the National Academy of Sciences · 1972 · 6.9K citations

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Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Don W. Cleveland, S G Fischer, Marc W. Kirschner et al. · Journal of Biological Chemistry · 1977 · 5.6K citations · Full text

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