Detection of the major non‐serum derived proteins of body fluids by immunodeletion and two‐dimensional gel electrophoresis

Abstract

Abstract Commercial antisera to human serum were coupled to Sepharose 4B or Staphylococcus aureus bacteria and used as immunoadsorbents to separate the serum‐derived proteins in several types of body fluids from the fluid‐specific, non‐serum derived proteins. Two‐dimensional gel analysis of fluids after incubation with one or the other immunoadsorbent produced similar results. Cerebrospinal, prostatic and amniotic fluid each exhibited a characteristic array of probable fluid‐specific protein families which were not detected in serum. Data from model experiments suggested that Sepharose 4B was a somewhat better matrix material than Staphylococcus aureus cells. Pretreatment of body fluids with anti‐human serum immunoadsorbents should facilitate the detection of fluid‐specific protein markers of disease in two‐dimensional gels.