Development of a <scp>SCAR</scp> marker linked with a <scp>MYMV</scp> resistance gene in mungbean (<i><scp>V</scp>igna radiata</i> L. <scp>W</scp>ilczek)

Abstract

Abstract Yellow mosaic disease ( YMD ) caused by mungbean yellow mosaic virus ( MYMV ) is the most important disease of mungbean, causing great yield loss. The present investigation was carried out to study the inheritance and identify molecular markers linked with MYMV resistance gene by using F 1 , F 2 and 167 F 2 : 8 recombinant inbred lines ( RIL s) developed from the cross ‘ TM ‐99‐37’ (resistant) × M ulmarada (susceptible). The F 1 was susceptible, F 2 segregated in 3 S :1 R phenotypic ratio and RIL s segregated in 1 S :1 R ratio in the field screening indicating that the MYMV resistance gene is governed by a single recessive gene. Of the 140 RAPD primers, 45 primers showing polymorphism in parents were screened using bulked segregant analysis. Three primers amplified specific polymorphic fragments viz. OPB ‐07 600, OPC ‐06 1750 and OPB ‐12 820 . The marker OPB ‐07 600 was more closely linked (6.8 cM) with a MYMV resistance gene as compared to OPC ‐06 1750 (22.8 cM) and OPB ‐12 820 (25.2 cM). The resistance‐specific fragment OPB ‐07 600 was cloned, sequenced and converted into a sequence‐characterized amplified region ( SCAR ) marker and validated in twenty genotypes with different genetic backgrounds.