Journal of the American Chemical Society · 2007 · 45 citations · 8 references
Natural SciencesBiophysicsMolecular BiologyBiophotonicsBiophysical ChemistryMolecular ImagingCellular ImagingFluorescence ImagingFluorescence MicroscopyFluorescence SpectroscopyMolecular Probe DevelopmentPhotophysics Of Fluorescent ProbesActivatable Imaging ProbesCellular DynamicsFluorescence ProbesFluorescent DyesFluorescent Probe DesignFluorescent ProbesFluorophore DesignFret Imaging
Photoactivatable fluorophores are powerful imaging probes for tracking molecular and cellular dynamics with high spatiotemporal resolution. We have designed and synthesized a new caged FRET dye, CCC-1, comprising a caged coumarin and a water soluble xanthene dye. CCC-1 can be efficiently photolyzed by ultraviolet light. Its photolyzed product, CC-1, emits strong green fluorescence with a FRET efficiency of 95% when excited near 410 nm. The uncaging cross section of CCC-1 is about 6000 M -1 cm -1 at 365 nm, more than a hundred times higher than caged fluoresceins developed previously. The probe is also biocompatible and has built-in functionalities for bioconjugation.
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Radioactive Labeling of Antibody: A Simple and Efficient Method
D.J. Hnatowich, W.W. Layne, R.L. Childs et al. · Science · 1983 · 408 citations
Hideo Takakusa, Kazuya Kikuchi, Yasuteru Urano et al. · Chemistry - A European Journal · 2003 · 128 citations