Lipase Inhibition and Antiobesity Effect of Atractylodes lancea

Abstract

The ethanol extract of <i>Atractylodes lancea</i> rhizome displayed significant lipase inhibition with an IC₅₀ value of 9.06 µg/mL in a human pancreatic lipase assay from high-throughput screening. Bioassay-guided isolation led to the identification of one new polyacetylene, <i>syn-</i>(5<i>E</i>,11<i>E</i>)-3-acetoxy-4-O-(3-methylbutanoyl)-1,5,11-tridecatriene-7,9-diyne-3,4-diol (<b>7</b>), along with six known compounds (<b>1</b>–<b>6</b>). The structure of compound <b>7</b> was determined based on the analysis of NMR and MS data. Among these seven lipase inhibitors, the major compound atractylodin (<b>1</b>) showed the highest lipase inhibitory activity (IC₅₀ = 39.12 µM). The antiobesity effect of the ethanol extract of <i>Atractylodes lancea</i> rhizome was evaluated in a high-fat diet-induced obesity mice model at daily dosages of 250 mg/kg and 500 mg/kg body weight for 4 weeks, and treatment with this extract demonstrated a moderate efficacy at the 500 mg/kg dose level.