Visualizing Tyrosine Kinase Activity with Bipartite Tetracysteine Display

Abstract

BiAs binding: Recently we reported that the linear tetracysteine sequence preferred by FlAsH and ReAsH could be split between two members of a protein partnership or regions of a folded protein, while maintaining high affinity and brightness. Here we show that this tool—bipartite tetracysteine display—facilitates the design of E2, an encodable, site-selective, Src-family kinase sensor.