Determination of Norfloxacin in Chicken Tissues by HPLC with Fluorescence Detection

Abstract

An HPLC method for the determination of norfloxacin in tissues (muscle, liver, and fat) of chickens is described. The supernatant from a centrifuged homogenate was analyzed using an RP‐18 column with RP‐18 precolumn. Fluorescence at an excitation wavelength of 278 nm and an emission wavelength of 456 nm was used for detection. The mobile phase was water/methanol (65:35, v/v adjusted to pH 3 with H3PO4), which contained tetrabutylammonium hydrogen sulphate as ion‐pairing agent. The flow rate was 1 mL/min. The detection limit of norfloxacin in the tissues was 2.5 ng/mL of homogenate.