Biotechnology Progress · 2012 · 75 citations · 23 references
Life SciencesCell BiologyNeuroscienceElectromagneticsElectromagnetismStem Cell BiologyNervous TissueNeural DevelopmentNeural DifferentiationNeural InductionNeural Stem Cell BiologyNeural Stem CellsDifferentiationImmunofluorescence ImagingNeural Cell DifferentiationNeural Progenitor DifferentiationNeural Stem Cell DifferentiationNeural Stem Cell MarkersPluripotent Stem Cell Neural InductionStem Cell Marker Expression
Adult stem cells are considered multipotent. Especially, human bone marrow-derived mesenchymal stem cells (hBM-MSCs) have the potential to differentiate into nerve type cells. Electromagnetic fields (EMFs) are widely distributed in the environment, and recently there have been many reports on the biological effects of EMFs. hBM-MSCs are weak and sensitive pluripotent stem cells, therefore extremely low frequency-electromagnetic fields (ELF-EMFs) could be affect the changes of biological functions within the cells. In our experiments, ELF-EMFs inhibited the growth of hBM-MSCs in 12 days exposure. Their gene level was changed and expression of the neural stem cell marker like nestin was decreased but the neural cell markers like MAP2, NEUROD1, NF-L, and Tau were induced. In immunofluorescence study, we confirmed the expression of each protein of neural cells. And also both oligodendrocyte and astrocyte related proteins like O4 and GFAP were expressed by ELF-EMFs. We suggest that EMFs can induce neural differentiation in BM-MSCs without any chemicals or differentiation factors.
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Federica I. Wolf, Angela Torsello, Beatrice Tedesco et al. · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · 2004 · 314 citations · Full text