Effect of Oxygen Concentration, Culture Format and Donor Variability on <i>in vitro</i> Chondrogenesis of Human Adipose Tissue-Derived Stem Cells

Abstract

The micropellet cultures at ambient or 15% oxygen concentration provided the most suitable environment for inducing chondrogenesis in ASCs. Furthermore, in light of the fact that the induction appeared in a zone-dependent manner, this format lends itself as a suitable model for further analysis of the relationship between chondrogenic differentiation and the gradient of nutrients.