Langmuir · 2003 · 62 citations · 11 references
Natural SciencesMaterials EngineeringMaterials SciencePolymer ChemistryPolymer MaterialsSurface SciencePolymer SurfacesPolymer Thin FilmsSurface HydrophilicitySurface HydrophobicitySurface ModificationSurface Modification Of BiomaterialsAttenuated Total ReflectanceHydrophilic Surface CoatingsPlasma PretreatmentPlasma-assisted GraftingPolymer GraftingPolymer Grafting Onto SurfacesSurface GraftingSurface Hydrophilization
We report a method to prepare hydrophilic poly(dimethylsiloxane) (PDMS) stamps that could transfer patterns of polar molecules homogeneously in soft lithography. In this method, we demonstrated a two-step procedure by using a mixed gas of argon (Ar) and hydrogen (H 2 )-based microwave plasma pretreatment to activate PDMS and subsequent acrylonitrile grafting onto PDMS, generating a hydrophilic surface with cyano (−CN) group coatings. The PDMS hydrophilic surface exhibited high affinity for wetting acetonitrile, which is a conventional solvent for DNA synthesis. Acetonitrile droplets possessed contact angles as low as 17 ± 7° on the cyano (−CN) groups grafted PDMS stamps surface. The hydrophilicity was stable and could last for at least 1 month at room temperature. Attenuated total reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectrum were used to characterize the surface hydrophilization. Such a hydrophilic and inert PDMS stamp showed significance for reactive multilevel fabrications in soft lithography. The possible chemical mechanism for PDMS surface grafting was discussed.
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Patterning Mammalian Cells Using Elastomeric Membranes
Emanuele Ostuni, Ravi S. Kane, Christopher S. Chen et al. · Langmuir · 2000 · 314 citations
Thymocyte Expression of RAG-1 and RAG-2: Termination by T Cell Receptor Cross-Linking
Laurence A. Turka, David G. Schatz, Marjorie A. Oettinger et al. · Science · 1991 · 261 citations