Chemotaxis of monocytes and neutrophils to platelet-derived growth factor.

TLDR

PDGF is chemotactic for monocytes and neutrophils. PDGF induces monocyte chemotaxis comparable to C5a at 20 ng/ml and neutrophil chemotaxis at 60 % of C5a at 1 ng/ml, with activity blocked by anti‑PDGF antisera and protamine, whereas EGF shows no effect, indicating PDGF may recruit inflammatory cells to platelet‑rich sites.

Abstract

The platelet-derived growth factor (PDGF) is shown to be chemotactic for monocytes and neutrophils. Maximum monocyte chemotaxis to PDGF is fully equal to that achieved with C5a and occurs at congruent to 20 ng/ml (congruent to 0.7 nM). Maximum neutrophil chemotaxis is congruent to 60% that achieved with C5A but occurs at congruent to 1 ng/ml (congruent to 32 pM). The chemotactic activity of PDGF is blocked by specific antisera to PDGF and by protamine sulfate, a competitive inhibitor of PDGF binding to cell surfaces. In contrast to PDGF, epidermal growth factor shows no chemotactic activity for inflammatory cells at 0.5-100 ng/ml. The high level of chemotactic activity of PDGF suggests that in addition to its role as a mitogen for smooth muscle cells and fibroblasts, PDGF may be involved in attracting inflammatory cells to sites of platelet release.