Synthesis of pppGpN typs dinucleotide derivatives: the 5′ end sequence of some RNAs

Abstract

A rapid and simple synthesis of pppGpN type /N equals C, U or A/ diribonucleotide derivatives is described by coupling guanosine 2', 3'-cyclic phosphate 5'-triphosphate with the appropriate ribonucleoside in the presence of ribonuclease T-1.