SPOT Synthesis of Peptide Arrays on Self‐Assembled Monolayers and their Evaluation as Enzyme Substrates

Abstract

Sweet SPOT: An efficient SPOT peptide synthesis methodology on self-assembled monolayer-coated gold surfaces was developed. Arrays of peptides and glycopeptides were subsequently used to evaluate substrate specificity of proteases and glycosyltransferases. The efficiency of all reactions could be easily monitored directly on-chip by using MALDI-ToF MS. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.