Dimethylallylpyrophosphate:3,9‐dihydroxypterocarpan 10‐dimethylallyl transferase from <i>Phaseolus vulgaris</i> Identification of the reaction product and properties of the enzyme

Abstract

A microsomal fraction of elicitor‐challenged bean ( Phaseolus vulgaris ) cell suspension cultures catalyses the prenylation of 3,9‐dihydroxypterocarpan (DHP) to 3,9‐dihydroxy‐10‐dimethylallylpterocarpan (phaseollidin) with dimethylallylpyrophosphate (DMAPP) as the prenyl donor. K m values for DMAPP and DHP are in the range 1–3 μM. Strong product inhibition with phaseollidin and phaseollin is observed.