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SOLID-PHASE RADIOIMMUNOASSAY OF ESTROGENS IN BIOLOGICAL FLUIDS
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1970
Year
GynecologyFemale Reproductive FunctionReproductive BiologyPregnancy SerumBioanalysisAnalytical ChemistryClinical ChemistryPublic HealthE 2BiophysicsChromatographyHormonal ReceptorEndocrinologyPharmacologyOvarian HormoneEndocrine DisruptorsDried Ether ExtractMedicineEndocrine Research
ABSTRACT Using antibody-coated test tubes as a detection method, three types of assay for 17β-estradiol (E 2 ) have been investigated. Method A involved the measurement of E 2 in the dried ether extract of biological fluids. Method B required a chromatographic step after ether extract, prior to measurement of E 2 . Method C (direct method) measured E 2 directly in the biological fluid, diluted with assay buffer. Method C was found reliable for the measurement of E 2 in human follicular fluid, but not in pregnancy serum. Method A was adequate for pregnancy serum, but overestimated E 2 in serum from normal adult male and female subjects. Method B was reliable to measure E 2 in all the biological fluids tested.