Design and Efficient Soluble Expression of a Sweet Protein, Brazzein and Minor-Form Mutant

Abstract

Figure 1. The DNA sequence of the synthesized brazzein gene encoding 53 amino acids that combined with the pelB leader sequence and amino acid sequence of the resulted recombinant brazzein. The sequence ends with two TAG and TAA termination codons. Gray box ( ) indicates the sequence of the pelB leader sequence.