Usage of a DNA Aptamer as a Ligand Targeting Microcystin

Abstract

Abstract Microcystin is a bacterial hepatotoxin generated from the cyanobacteria causing waterblooms. A DNA aptamer binding to microcystin was screened by the in vitro selection method of twelve rounds in order to obtain a sorbent suitable for a microcystin detection system. The obtained DNA aptamer showed specific binding to microcystin. The direct detection range using an immobilized aptamer and a surface plasmon resonance biosensor was 50 μ/ml to 1000 μ/ml. Key Words: DNA aptamer in vitro selectionmicrocystinsurface plasmon resonance