FRACTIONATION OF HUMIC ACID BY GEL PERMEATION CHROMATOGRAPHY

Abstract

Summary Humic acids from the L layer and B 2h horizons of a humus‐iron podzol were fractionated by gel permeation chromatography. Elution of the gel column with distilled water adjusted to pH 7.5 gave a fractionation based on both gel permeation and adsorption chromatography. This procedure gave filtered products containing most of the organic nitrogen, and an absorbed fraction. The latter contained most of the components which gave benzene carboxylic acids on oxidation of the humic acid with dilute nitric acid.