Phytopathology · 1993 · 57 citations · 0 references
Life SciencesGeneticsPlant SciencesBreeding For Disease ResistanceGenomicsPlant GeneticsGene MappingGenetic MappingMolecular Markers In PlantsPest Resistance GeneticsQuantitative Trait LociQuantitative Trait Loci MappingBackcross GenerationsBackcross Population DesignGenetic Mapping PopulationsMolecular Markers For ResistanceResistance Gene Qtl MappingResistance Locus CharacterizationRestriction Fragment Length Polymorphism AnalysisRestriction Fragment Length Polymorphism Mapping
To facilitate understanding of the molecular basis of Htn1 resistance in maize to northern corn leaf blight, we mapped the Htn1 locus by restriction fragment length polymorphism (RFLP) analysis. Linkage with the Htn1 locus, using a backcross population, was initially detected with the RFLP probe UMC16, which hybridized to two loci: UMC16A on the long arm of chromosome three and the unmapped locus UMC16B. Independent assortment of Htn1 with UMC16A and linkage to UMC16B was verified with RFLP markers closely flanking UMC16A on the long arm of chromosome three. Information obtained from previous studies, detailing duplicate loci in maize, indicated that RFLP loci in the region around UMC16A are duplicated on the long arm of chromosome eight in the region of Idh1 [...]