An osmotic induction method for externally marking saltwater fishes, <i>Stigmatopora argus</i> and <i>Stigmatopora nigra</i>, with calcein

Abstract

Calcein marking via osmotic induction and overnight immersion was compared in saltwater fishes. Immersion in a salt solution for 10 min followed by 30 min in a 500 mg l −1 calcein solution produced the highest fluorescent mark in 40 min with no effect on survival.