Multiplex Sequencing of Seven Ocular Herpes Simplex Virus Type-1 Genomes: Phylogeny, Sequence Variability, and SNP Distribution

Abstract

Multiplex sequencing is a cost-effective way to obtain the genomic sequences of ocular HSV-1 isolates with sufficient coverage of the unique regions for genomic analysis. The number of SNPs and their distribution will be useful for analyzing the genetics of virulence, and the sequence data will be useful for studying HSV-1 evolution and for the design of structure-function studies.