Solid‐State NMR of Matrix Metalloproteinase 12: An Approach Complementary to Solution NMR

Abstract

Data transfer. The solid-state proton-driven spin diffusion (PDSD) and J-decoupled PDSD NMR spectra of the microcrystalline catalytic domain of matrix metalloproteinase 12 (MMP-12, 17 kDa) have been recorded. It is shown that such spectra can be largely assigned in a few days by using the available liquid-state assignment and validated with an independent sequential assignment based on 3D NCACX and NCOCX PDSD experiments. This demonstrates how quickly the liquid-state assignment of comparably large protein can be transferred to the solid state. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2007/z600408_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.