The Glomerular Permeability Determined by Dextran Clearance Using Sephadex Gel Filtration

TLDR

The study introduces a method to assess glomerular permeability by measuring dextran clearance via Sephadex gel filtration. The method uses Sephadex gel filtration to separate dextran molecules and quantify their clearance rates. Clearance decreases with increasing molecular weight, being near zero above 55,000 Da and averaging 95 ml/min at 15,000 Da, with a linear relationship to molecular weight on a log–log plot and to Stokes radius on a semilog plot, though the latter shows a slight deviation at high molecular weight. No additional metadata provided.

Abstract

A method is described for measuring glomerular permeability by dextran clearance using Sephadex gel filtration technique. Clearance is shown to be a function of molecular size, decreasing with increasing molecular weight. At a mol. weight higher than 55,000, the clearance is very low and approximately zero, while it averages 95 ml per minute at a mol. weight of 15,000. If plotted in a log.log. graph, a linear relation is found between clearance and molecular weight. In a semilog. graph, however, with clearance plotted against log. Stokes radius, the relation is also linear, with a slight deflection in the high molecular part, however.