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Comparison of the Unit-chain Distributions of Glycogens from Different Biological Sources, Revealed by Anion Exchange Chromatography
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1993
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EngineeringPolysaccharideFood ChemistryBiosynthesisGas ChromatographyBioanalysisAnion Exchange ChromatographyAnalytical ChemistryLiquid ChromatographyDifferent Biological SourcesChromatographyGlycosylationBiochemistryUnit-chain DistributionsOyster GlycogenQuantitative HpaecChromatographic AnalysisPharmacologyMost GlycogensMass SpectrometryMicrobiologyMedicineCarbohydrate-protein InteractionDrug Analysis
The precise distributions of α-1,4-unit-chains of several kinds of glycogens, average chain length 7–13, from different sources, e.g., mammals, shellfish, mushrooms, and microorganisms, were compared using high performance anion exchange chromatography (HPAEC). These glycogens were completely debranched, and a series of maltosaccharides derived from individual unit-chains were analyzed. Most glycogens had similar chromatographic profiles, with degrees of polymerization (DPs), from 4–5 to 35; oyster glycogen contained shorter chains of DP 2–3. On the other hand, molar-based distribution patterns of these glycogens appeared to be specific to their origins. Ratios of their A- to B-chains varied from 0.6: 1 to 1.2: 1, as estimated by quantitative HPAEC of maltosaccharides by debranching of the β-limit dextrins. Possible chain lengths of the exterior A-chains were also discussed.