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Plasma 11-Deoxycorticosteroid and ACTH Response to Metyrapone (Plasma Metyrapone Test)
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1969
Year
Adrenal GlandBioanalysisMetyrapone TestToxicologyAnalytical ChemistryClinical ChemistryHuman MetabolismSteroid MetabolismChromatographyHealth SciencesPlasma Metyrapone TestTherapeutic Drug MonitoringChemical PathologyActh DeficiencyEndocrinologyPharmacologyPhysiologyClinical PharmacologyMetabolismMedicineOral MetyraponePharmacokineticsDrug Analysis
The plasma concentration of 11-deoxycorticosteroids (11-deoxy CS) was measured by competitive protein-binding after extraction with dichloromethane and partitioning of the extract between benzene and water. When normal subjects were given metyrapone orally, plasma 11-deoxy CS levels rose from 2.0 or less to 12–32 μg/100 ml; plasma ACTH concentrations (radioimmunoassay) rose concomitantly from 0–5 to 8–11 ng/100 ml. When metyrapone was given intravenously to normal subjects, plasma 11-deoxy CS levels rose from less than 2.0 to 9–19 μg/100 ml. Plasma ACTH levels also increased. In patients with ACTH deficiency the plasma concentrations of 11-deoxy CS were 0–7 μg/100 ml after oral metyrapone. The plasma 11-deoxy CS method was found to be convenient and easy to perform. The usefulness of the method as a routine diagnostic procedure when applied to the metyrapone test was demonstrated.