Journal of Endocrinology · 1956 · 128 citations · 0 references
MedicineBiochemistryEndocrinologyCompartmental ModelingDiabetologyNeuroendocrinologyDiabetes MellitusGlucose HomeostasisDose-response Curve AnalysisDose-response Curve FittingDose-response Curve ModelingInsulin MeasurementInsulin PhysiologyLog-dose ResponseSkeletal Muscle Glucose MetabolismSkeletal Muscle Glucose Uptake
SUMMARY An in vitro method of insulin assay based upon the glucose uptake of the isolated rat diaphragm is described and a statistical appraisal of the method given. The minimum concentration of insulin which was detected by this method was of the order of 10 −4 i.u. of insulin/ml. The index of precision (λ) varied in eighteen studies of regression from 0·18 to 0·74. The slope of the log dose-response curve was found to vary significantly from experiment to experiment. The results are briefly compared with those obtained with other in vitro methods of insulin assay.