Analytical Chemistry · 1997 · 92 citations · 7 references
Life SciencesBiochemistryMolecular BiologyAnalytical ChemistryMass Spectrometric AnalysisProteomicsMass SpectrometryMass Spectrometry-based ProteomicsPeptide AnalysisProtein CharacterizationProtein ElectrophoresisProtein SeparationGel ElectrophoresisMass Spectrometry ProteomicsMass Spectrometry-based Protein IdentificationPlasma Protein ElectrophoresisProtein DetectionProtein Mass SpectrometryProtein Separation TechniquesTwo-dimensional Gel Electrophoresis
A novel approach is reported for the analysis and identification of proteins separated by 2D-PAGE with scanning infrared matrix-assisted laser desorption/ionization mass spectrometry (scanning IR-MALDI-MS). The proteins of human blood plasma were separated by 2D-PAGE, electroblotted onto PVDF membranes, incubated in matrix solution, and then scanned by IR-MALDI-MS. Mass contour plots of selected spots were obtained. Protein separation is shown to be conserved by comparison with silver-stained gels. The sensitivity for the protein detection is comparable if not better than that of silver-stained gels. Posttranslational modifications were identified by comparing the measured mass to the one calculated from the known DNA sequence. Adduct formation to unprotected cysteine residues during gel separation is demonstrated for selected proteins.
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Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry
Matthias Wilm, Tony Houthaeve, Stephen Breit et al. · Nature · 1996 · 1.7K citations
Plasma protein map: An update by microsequencing
Graham J. Hughes, Séverine Frutiger, Nicole Paquet et al. · Electrophoresis · 1992 · 135 citations