Development of microsatellite markers in the Japanese pear (<i>Pyrus pyrifolia</i> Nakai)

Abstract

Abstract Thirteen polymorphic microsatellite loci were developed in the Japanese pear ( Pyrus pyrifolia Nakai) by using an enriched genomic library. The obtained microsatellite loci showed a high degree of polymorphism in the Japanese pear with 3–6 alleles per locus. The average values of observed and expected heterozygosities among these 13 loci were 0.69 and 0.71, respectively. Ten microsatellites could successfully amplify loci in the European pear ( Pyrus communis L.), which were highly polymorphic as well.