1c:I[419806,["/_next/static/chunks/bb923814e1f4d2e9.js","/_next/static/chunks/dd3ee9f5b7716ca6.js","/_next/static/chunks/4204a0ae82542ca8.js","/_next/static/chunks/1270dead247da766.js","/_next/static/chunks/6e1aa89a642f40be.js","/_next/static/chunks/2d7aa753e81324a8.js","/_next/static/chunks/8ffd0b71567920d8.js","/_next/static/chunks/67ea6dfd21f16c5f.js","/_next/static/chunks/24eb4379f68013fd.js","/_next/static/chunks/b8cd775ffdf935bd.js","/_next/static/chunks/983103cd9eb7b428.js","/_next/static/chunks/263f2cdcb9aa2341.js","/_next/static/chunks/294b9525248c8e5f.js"],"default"] 1d:T48a,A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quantitative transfer of ribosomal proteins from gels containing urea. For sodium dodecyl sulfate gels, the original band pattern was obtained with no loss of resolution, but the transfer was not quantitative. The method allows detection of proteins by autoradiography and is simpler than conventional procedures. The immobilized proteins were detectable by immunological procedures. All additional binding capacity on the nitrocellulose was blocked with excess protein; then a specific antibody was bound and, finally, a second antibody directed against the first antibody. The second antibody was either radioactively labeled or conjugated to fluorescein or to peroxidase. The specific protein was then detected by either autoradiography, under UV light, or by the peroxidase reaction product, respectively. In the latter case, as little as 100 pg of protein was clearly detectable. It is anticipated that the procedure will be applicable to analysis of a wide variety of proteins with specific reactions or ligands.1e:T48c,Antagonism of ectotrophic mycorrhizal fungi to Phytophthora cinnamomi, other root pathogenic fungi, and soil bacteria was examined. In agar plate tests, Laccaria laccata, Lactarius deliciosus, Leucopaxillus cerealis var. piceina, Pisolithus tinctorius, and Suillus luteus inhibited growth of nearly half of the 48 different fungal root pathogens. Leucopaxillus cerealis var. piceina inhibited 92% of the test pathogens. Differences in sensitivity of several isolates of P. cinnamomi to inhibitions by this symbiont were not found. Culture filtrates of L. cerealis var. piceina were inhibitory also to growth of P. cinnamomi and soil bacteria. Zoospore germination was inhibited completely in filtrates of this symbiont. Maximum antibiotic production occurred during the rapid growth phase in liquid culture. Length of culture incubation and temperature strongly influenced production of inhibitory substances by L. cerealis var. piceina in liquid culture. It grew best from 10 to 20 C, whereas P. tinctorius grew best from 30 to 35 C in liquid culture.

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