Targeting of a foreign protein into the thylakoid lumen of pea chloroplasts

Abstract

A chimaeric gene was constructed which encodes the pre‐sequence of the 33 kDa protein of the oxygen‐evolving complex, a thylakoid lumen protein, linked to dihydrofolate reductase, a cytoplasmic protein. The encoded fusion protein is transported into the thylakoid lumen of isolated pea chloroplasts, with concomitant removal of the pre‐sequence.