Cytogenetics and Cell Genetics · 2008 · 73 citations · 0 references
Life SciencesGeneticsMolecular GeneticsCytogeneticsHuman GeneticsMedical GeneticsChromosome MappingGene MappingGenetic MappingMendelian InheritanceAlpha Satellite DnaChromosome PolymorphismDna In Situ HybridizationGenetic Marker MappingRestriction Fragment Length Polymorphism AnalysisRestriction Fragment Length Polymorphism MappingRestriction Site PolymorphismSomatic Cell Hybrid Mapping
The isolation and localization of a chromosome 12-specific alpha satellite DNA sequence, p alpha 12H8, is described. This clone contains a complete copy of the 1.4-kb HindIII higher-order repeat present within the alpha satellite array on chromosome 12. The specificity of p alpha 12H8 was demonstrated by in situ hybridization and Southern blot analysis of a somatic cell hybrid mapping panel, both performed under high-stringency conditions. Polymorphic restriction patterns within the alpha satellite array, revealed by the use of the restriction enzymes BglII and EcoRV, were demonstrated to display Mendelian inheritance. These properties make p alpha 12H8 a valuable genetic marker for the centromeric region of chromosome 12.