Concepedia

Performance of a Commercial, Type-Specific Enzyme-Linked Immunosorbent Assay for Detection of Herpes Simplex Virus Type 2-Specific Antibodies in Ugandans

Oliver Laeyendecker, Charla Henson, Ronald H. Gray, Ruby H.N. Nguyen, Bobbi Jo Horne, Maria J. Wawer, David Serwadda, Noah Kiwanuka, Rhoda Ashley Morrow, Wayne Hogrefe,

Journal of Clinical Microbiology · 2004 · 66 citations · 9 references

DOI

Concepts

Abstract

Two hundred forty-eight human immunodeficiency virus (HIV)-positive and 496 HIV-negative subjects in Uganda were tested by HerpeSelect herpes simplex virus type 2 enzyme-linked immunosorbent assay (ELISA) and Western blotting to optimize the ELISA for use in this population. A higher index cutoff value was required for optimal sensitivity and specificity, and overall performance of the assay was not affected by HIV status.

References

9

Receiver-operating characteristic (ROC) plots: a fundamental evaluation tool in clinical medicine

M H Zweig, Gregory Campbell · Clinical Chemistry · 1993 · 6.6K citations

, , +16

Probability of HIV-1 transmission per coital act in monogamous, heterosexual, HIV-1-discordant couples in Rakai, Uganda

Ronald H. Gray, Maria J. Wawer, Ron Brookmeyer et al. · The Lancet · 2001 · 1.3K citations

, , +13

Comparison of Western blot (immunoblot) and glycoprotein G-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera

R Ashley, Julie Militoni, F Lee et al. · Journal of Clinical Microbiology · 1988 · 540 citations · Full text

, , +15

Genital Herpes and Public Health

Lawrence Corey, H. Hunter Handsfield · JAMA · 2000 · 235 citations

, , +15

Detection of Herpes Simplex Virus Type 2-Specific Immunoglobulin G Antibodies in African Sera by Using Recombinant gG2, Western Blotting, and gG2 Inhibition

Wayne Hogrefe, Xin Su, James Song et al. · Journal of Clinical Microbiology · 2002 · 82 citations

, , +11