Some properties of diastereomers formed in the reactions of N-ethylmaleimide with biological thiols.

Abstract

TV-Substituted maleimides which are colored, fluorescent or bifunctional have been widely used as alkylating agents for biologically important thiols.1 ~4) The resulting diastereomers makethe profile of their chromatograms complicated, as shown by Smyth et al.5) This paper describes the separation of the diastereomers of thiol-TV-