A sensitive dual signal amplification method for western blotting based on antibody-functionalised graphene oxide and gold nanoparticles

Abstract

In this study, we report an ultrasensitive western blotting method using antibody-functionalised graphene oxide sheets and gold nanoparticles. Additionally, the cost is reduced greatly by conjugating two different primary antibodies on gold nanoparticles (C. Welinder and L. Ekblad, J. Proteome Res., 2011, 10, 1416-1419).