Characterization of VIP‐sensitive adenylate cyclase in guinea pig brain

Abstract

This paper demonstrates that VIP activates an adenylate cyclase from a synaptosomal fraction of guinea pig brain. This activation was not potentiated by guanyl triphosphate nucleotides, and was unaffected by α- and β-adrenergic blockers and by atropine. Furthermore, peptides related to VIP, like secretin, glucagon and somatostatin, were devoid of significant agonistic or antagonistic activity. EGTA was also without effect on basal and VIP stimulated activities while calcium at concentrations higher than 10-5 M inhibited both activities.